How To Make Biorelevant Media (FaSSIF, FeSSIF) Fast!
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How to prepare Biorelevant Media (FaSSIF, FeSSIF) from Phares SIF Powder
Preparing Biorelevant Media (FaSSIF or FeSSIF) from SIF Powder is a very easy 2-step process:
1) Prepare the buffer
2) Add the SIF Powder
Such a simple method enables you to freshly produce any volume of Biorelevant Media required in just minutes. One 56g bottle of SIF Powder produces 5L of FaSSIF or 25L of FeSSIF.
Download our SIF Powder Preparation Protocol to find out more: click here
How To Store SIF Powder
SIF Powder should be stored at between 2-8°C and protected from light and humidity. Before opening the bottle, equilibrate to room temperature. The expiry date, stated on the accompanying Certificate of Analysis, is 18 months.
Tips For Getting The Best Results From Your FeSSIF/FaSSIF Media
At Phares, we are continually conducting research into the field of Biorelevant Media for industrial application. It is part of our customer care policy to update our customers with this information so you can benefit from our latest findings.
*Use HPLC as your analytical tool*
HPLC or specialist UV equipment is recommended as an analytical tool for more accurate quantitative drug analysis in the presence of FaSSIF and FeSSIF media to eliminate possible UV interference.
*Make FaSSIF and FeSSIF freshly to reduce the risk of microbial spoilage or physicochemical changes*
As FaSSIF and FeSSIF are so quick and easy to prepare using SIF Powder you can make it freshly, eliminating the need to store the media.
Tips For Preparing FaSSIF and FeSSIF Media With Reproducible Physical Properties
*Equilibrate FaSSIF media to obtain a stable particle size*
We recommend in our Preparation Protocol equilibrating this media for 2 hours after preparation before use to obtain a stable particle size.
*Avoid storing FaSSIF media in the refrigerator*
We suggest avoiding storage in the refrigerator as this can change the particle size of FaSSIF.
To read these preparation tips in greater
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